The utilization of bacteria based biosensors for the study of toxic compounds is regarded as a key advancement for rapid and reliable detection of hazards for health and environment, such as the use of bamboo composite products in homebuilding. The purpose of this study was to test the toxicity of bamboo (Phyllostachys edulis) powders, both pure and treated with epoxidic resins, using the engineered bioluminescent Escherichia coli, TV 1061, sensitive to protein damage, and Escherichia coli DPD 2794, sensitive to DNA damage. Other elements of known toxicity were tested, to create a standard for comparison. Different protocols for the interaction between bacteria and toxic agent with different concentrations were tested. The bioluminescent signal given off by the bacteria was analysed with luminometer and normalized as induction factor. Scanning electron microscope was used to evaluate cell surface modifications. For both strains the luminescence damage response followed a peculiarly shaped curve.

Bioluminescent engineered Escherichia coli strains for detection of bamboo powders toxicity

MANZANO, Marisa;
2015-01-01

Abstract

The utilization of bacteria based biosensors for the study of toxic compounds is regarded as a key advancement for rapid and reliable detection of hazards for health and environment, such as the use of bamboo composite products in homebuilding. The purpose of this study was to test the toxicity of bamboo (Phyllostachys edulis) powders, both pure and treated with epoxidic resins, using the engineered bioluminescent Escherichia coli, TV 1061, sensitive to protein damage, and Escherichia coli DPD 2794, sensitive to DNA damage. Other elements of known toxicity were tested, to create a standard for comparison. Different protocols for the interaction between bacteria and toxic agent with different concentrations were tested. The bioluminescent signal given off by the bacteria was analysed with luminometer and normalized as induction factor. Scanning electron microscope was used to evaluate cell surface modifications. For both strains the luminescence damage response followed a peculiarly shaped curve.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11390/1097226
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