This chapter focuses on several different levels of culture and their effect on project organizations operating in the IT sector in Hungary and Italy. The research study in this chapter aims to reveal whether sectoral or national culture has a stronger effect on these project organizations and their preferred culture profiles. The results of the study show that the IT sector in both countries is characterized by a culture profile of Clan domination, but there are also differences between the preferred culture profiles of the project organizations in both countries due to one national cultural dimension (which is masculinity) and thus the effect of national culture is stronger than the effect of sectoral culture of the IT sector.