The role of natural humic substances on plant cell phosphate metabolism was investigated in tobacco BY-2 suspension cell cultures grown in the presence of different humic samples (HS). These HS were characterized by CPMAS-NMR spectroscopy and on-line pyrolysis-gas chromatography-mass spectrometry. After incubation of suspension cell cultures for 7 days, either in the presence or absence of HS, cells were facing Pi starvation. The most hydrophilic and smallest in molecular size among fractions of HS (fraction III) induced a partial relief from Pi starvation, increasing total cell phosphate amount, ATP and glucose-6-phosphate levels, as well as the activity of secreted acid phosphatases. Fraction III induced also a decrease of KCN-insensitive respiration, evaluated in both suspension cells and isolated mitochondria. The molecular characteristics of fraction III are conducive to a flexible conformational structure that may induce an efficient release of Pi from organic sources (e.g. nucleic acids), released in the media by damaged or dead cells, exerting a sort of positive effect on either the production or activity of extracellular Pi hydrolytic enzymes.

PHOSPHATE LEVEL AND ENERGETIC METABOLISM OF TOBACCO BY- 2 SUSPENSION CELL CULTURES TREATED WITH HUMIC ACIDS.

ZANCANI, Marco;PETRUSSA, Elisa;KRAJNAKOVA, Jana;CASOLO, Valentino;MACRI', Francesco Arturo;VIANELLO, Angelo
2008-01-01

Abstract

The role of natural humic substances on plant cell phosphate metabolism was investigated in tobacco BY-2 suspension cell cultures grown in the presence of different humic samples (HS). These HS were characterized by CPMAS-NMR spectroscopy and on-line pyrolysis-gas chromatography-mass spectrometry. After incubation of suspension cell cultures for 7 days, either in the presence or absence of HS, cells were facing Pi starvation. The most hydrophilic and smallest in molecular size among fractions of HS (fraction III) induced a partial relief from Pi starvation, increasing total cell phosphate amount, ATP and glucose-6-phosphate levels, as well as the activity of secreted acid phosphatases. Fraction III induced also a decrease of KCN-insensitive respiration, evaluated in both suspension cells and isolated mitochondria. The molecular characteristics of fraction III are conducive to a flexible conformational structure that may induce an efficient release of Pi from organic sources (e.g. nucleic acids), released in the media by damaged or dead cells, exerting a sort of positive effect on either the production or activity of extracellular Pi hydrolytic enzymes.
File in questo prodotto:
File Dimensione Formato  
proceedings sifv 2008.pdf

non disponibili

Tipologia: Altro materiale allegato
Licenza: Non pubblico
Dimensione 972.34 kB
Formato Adobe PDF
972.34 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11390/694073
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact