Caspases are critical regulators of the apoptotic program, responsible for the harmonic dismantling of the cell. Cell death can occur by way of different options (necroptosis, necrosis, extreme autophagy) but once caspases are fully engaged it will take the apoptotic route. Hence, in general, caspase activity is inversely proportional to cell viability. Caspase activation can be measured by means of different techniques. Here, we describe three different methodologies to measure/observe caspase activation. Two approaches are recommended for studies on the whole cell population, whereas the third one is designed for visualizing caspase activation in single live cells.
Measurement of Caspase Activity: From Cell Populations to Individual Cells
BRANCOLINI, Claudio
2011-01-01
Abstract
Caspases are critical regulators of the apoptotic program, responsible for the harmonic dismantling of the cell. Cell death can occur by way of different options (necroptosis, necrosis, extreme autophagy) but once caspases are fully engaged it will take the apoptotic route. Hence, in general, caspase activity is inversely proportional to cell viability. Caspase activation can be measured by means of different techniques. Here, we describe three different methodologies to measure/observe caspase activation. Two approaches are recommended for studies on the whole cell population, whereas the third one is designed for visualizing caspase activation in single live cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
