The present invention relates to a method for determining the presence and/or amount of at least one analyte (T) that may be contained in a sample. The method comprises: (a) a recognition step during which said sample (E) is placed in the presence of at least one primary probe (1), in order to form, if necessary, a primary complex (C1), (b) a development step during which at least one developing reagent is added, capable of forming, starting from the primary probe (1) of said primary complex (C1), a secondary complex (C2), said at least one developing reagent comprising: - at least one amplification probe (3), of the core (31) - shell (32) type, wherein said shell (32) comprises several molecules of an affinity tag (321), and - a tracer (21) for converting the secondary complex (C2) into a visible and/or measurable signal, said tracer (21) being bound or intended to bind with the affinity tag (321) of said at least one amplification probe (3), so that in said secondary complex (C2) at least some of the molecules of said affinity tag (321) of said at least one amplification probe (3) are bound with said tracer (21).
METHOD AND SYSTEM FOR DETERMINING THE PRESENCE AND/OR AMOUNT OF AT LEAST ONE ANALYTE THAT MAY BE CONTAINED IN A SAMPLE
MANZANO MARISA;VIZZINI PRIYA
2021-01-01
Abstract
The present invention relates to a method for determining the presence and/or amount of at least one analyte (T) that may be contained in a sample. The method comprises: (a) a recognition step during which said sample (E) is placed in the presence of at least one primary probe (1), in order to form, if necessary, a primary complex (C1), (b) a development step during which at least one developing reagent is added, capable of forming, starting from the primary probe (1) of said primary complex (C1), a secondary complex (C2), said at least one developing reagent comprising: - at least one amplification probe (3), of the core (31) - shell (32) type, wherein said shell (32) comprises several molecules of an affinity tag (321), and - a tracer (21) for converting the secondary complex (C2) into a visible and/or measurable signal, said tracer (21) being bound or intended to bind with the affinity tag (321) of said at least one amplification probe (3), so that in said secondary complex (C2) at least some of the molecules of said affinity tag (321) of said at least one amplification probe (3) are bound with said tracer (21).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
