The objective of this trial was to determine the effect of breed and whole linseed addition on the expression of some genes involved in the lipid metabolism in subcutaneous adipose tissue of young bulls. Thirty-two young bulls were assigned to four groups following a factorial design 2 breeds (Italian Simmental, IS and Italian Holstein, IH) x 2 diets (containing 8% of DM of ground whole linseed, WL and without linseed, CON). At slaughter the 8th rib sample joint was taken and dissected with the aim to predict the carcass composition and samples of subcutaneous adipose tissue (head tail) were collected, from which RNA was extracted. The mRNA abundances of stearoyl-CoA desaturase (SCD), fatty acid synthetase (FAS), lipoprotein lipase (LPL), leptin (LEP), PPARγ (Peroxisome proliferator-activated receptors) were detected by real-time PCR. Changes in gene expression were calculated using the delta-delta Ct method. Two-way ANOVA was used to determine the effects of diets and breeds. In the LEP mRNA expression analysis, the estimated carcass fat was used as a covariate. Results showed that the inclusion of linseed, rich in C18:3 n-3, increased the expression of SCD gene (P<0.05), LPL gene (P<0.05), and tended to increase the expression of FAS gene (P=0.056) without affecting the LEP and PPARγ mRNA abundance in the adipose tissue of bulls. No breed effect was found on the gene expression, also the interaction diet x breed was considered, but it never reached a level of significance (P>0.05). In our trial the linseed supplementation reduces the gene expression of key lipogenic enzymes in subcutaneous adipose tissue of young bulls by a mechanism that does not involve the PPARγ regulation.
Effect of whole linseed addition on the expression of some lipid metabolism genes in the adipose tissue of Italian Simmental and Holstein young bulls
CORAZZIN, Mirco;BOVOLENTA, Stefano;SACCA', Elena;PIASENTIER, Edi
2011-01-01
Abstract
The objective of this trial was to determine the effect of breed and whole linseed addition on the expression of some genes involved in the lipid metabolism in subcutaneous adipose tissue of young bulls. Thirty-two young bulls were assigned to four groups following a factorial design 2 breeds (Italian Simmental, IS and Italian Holstein, IH) x 2 diets (containing 8% of DM of ground whole linseed, WL and without linseed, CON). At slaughter the 8th rib sample joint was taken and dissected with the aim to predict the carcass composition and samples of subcutaneous adipose tissue (head tail) were collected, from which RNA was extracted. The mRNA abundances of stearoyl-CoA desaturase (SCD), fatty acid synthetase (FAS), lipoprotein lipase (LPL), leptin (LEP), PPARγ (Peroxisome proliferator-activated receptors) were detected by real-time PCR. Changes in gene expression were calculated using the delta-delta Ct method. Two-way ANOVA was used to determine the effects of diets and breeds. In the LEP mRNA expression analysis, the estimated carcass fat was used as a covariate. Results showed that the inclusion of linseed, rich in C18:3 n-3, increased the expression of SCD gene (P<0.05), LPL gene (P<0.05), and tended to increase the expression of FAS gene (P=0.056) without affecting the LEP and PPARγ mRNA abundance in the adipose tissue of bulls. No breed effect was found on the gene expression, also the interaction diet x breed was considered, but it never reached a level of significance (P>0.05). In our trial the linseed supplementation reduces the gene expression of key lipogenic enzymes in subcutaneous adipose tissue of young bulls by a mechanism that does not involve the PPARγ regulation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
