Papa et al. engineer the CRISPR-Csy4 nuclease to localize into rotavirus viral factories and cleave (+)ssRNA replication intermediates, producing edits of the targeted dsRNA genome segment. This allows for the detection of secondary transcription-derived proteins made by the newly assembled viruses, demonstrating that they largely contribute to overall viral protein production.
CRISPR-Csy4-Mediated Editing of Rotavirus Double-Stranded RNA Genome
Petris G.
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2020-01-01
Abstract
Papa et al. engineer the CRISPR-Csy4 nuclease to localize into rotavirus viral factories and cleave (+)ssRNA replication intermediates, producing edits of the targeted dsRNA genome segment. This allows for the detection of secondary transcription-derived proteins made by the newly assembled viruses, demonstrating that they largely contribute to overall viral protein production.File in questo prodotto:
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