An enzyme linked immunosorbent assay (ELISA) was developed to detect the presence of Pasteurella piscicida. The test, after the preparation of the solid phase, takes less than 4 h to complete and the same technique can be applied for both bacteria in culture and in tissue. The limit of sensitivity for bacteria in culture was IO’ cells ml ’ or 10’ cells well ‘, if a plate reader is available or 10’ cells ml-’ (IO3 cells well- ‘> visually. Similar sensitivity was achieved (IO’ cells well-’ with a plate reader, IO’ cells well- ’ visually) when the ELISA was performed on tissue. The technique is specific and it offers a rapid, early and sensitive means of diagnosing Pasteurellosis during outbreaks and screening large populations of fish for the presence of P. piscicida. 0 1997 Elsevier Science B.V.

Development of an ELISA to detect Pasteurella piscicida in culture and in “spiked” fish tissue

VOLPATTI, Donatella;GALEOTTI, Marco;
1997-01-01

Abstract

An enzyme linked immunosorbent assay (ELISA) was developed to detect the presence of Pasteurella piscicida. The test, after the preparation of the solid phase, takes less than 4 h to complete and the same technique can be applied for both bacteria in culture and in tissue. The limit of sensitivity for bacteria in culture was IO’ cells ml ’ or 10’ cells well ‘, if a plate reader is available or 10’ cells ml-’ (IO3 cells well- ‘> visually. Similar sensitivity was achieved (IO’ cells well-’ with a plate reader, IO’ cells well- ’ visually) when the ELISA was performed on tissue. The technique is specific and it offers a rapid, early and sensitive means of diagnosing Pasteurellosis during outbreaks and screening large populations of fish for the presence of P. piscicida. 0 1997 Elsevier Science B.V.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11390/667378
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