An enzyme linked immunosorbent assay (ELISA) was developed to detect the presence of Pasteurella piscicida. The test, after the preparation of the solid phase, takes less than 4 h to complete and the same technique can be applied for both bacteria in culture and in tissue. The limit of sensitivity for bacteria in culture was IO’ cells ml ’ or 10’ cells well ‘, if a plate reader is available or 10’ cells ml-’ (IO3 cells well- ‘> visually. Similar sensitivity was achieved (IO’ cells well-’ with a plate reader, IO’ cells well- ’ visually) when the ELISA was performed on tissue. The technique is specific and it offers a rapid, early and sensitive means of diagnosing Pasteurellosis during outbreaks and screening large populations of fish for the presence of P. piscicida. 0 1997 Elsevier Science B.V.
Development of an ELISA to detect Pasteurella piscicida in culture and in “spiked” fish tissue
VOLPATTI, Donatella;GALEOTTI, Marco;
1997-01-01
Abstract
An enzyme linked immunosorbent assay (ELISA) was developed to detect the presence of Pasteurella piscicida. The test, after the preparation of the solid phase, takes less than 4 h to complete and the same technique can be applied for both bacteria in culture and in tissue. The limit of sensitivity for bacteria in culture was IO’ cells ml ’ or 10’ cells well ‘, if a plate reader is available or 10’ cells ml-’ (IO3 cells well- ‘> visually. Similar sensitivity was achieved (IO’ cells well-’ with a plate reader, IO’ cells well- ’ visually) when the ELISA was performed on tissue. The technique is specific and it offers a rapid, early and sensitive means of diagnosing Pasteurellosis during outbreaks and screening large populations of fish for the presence of P. piscicida. 0 1997 Elsevier Science B.V.File | Dimensione | Formato | |
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