The rapid diagnosis of apple proliferation mycoplasma-like organism, using polymerase chain reaction amplification of part of the 16S rRNA gene is reported. Apple proliferation MLO can be detected in as little as 0.3 g of diseased tissue. Results compare favourably with those obtained by other PCR-based methods. Operational speed, sensitivity, specificity and ease of performance of the method, with particular reference to indexing purposes, are discussed.
Rapid diagnosis of apple proliferation mycoplasma‐like organism using a polymerase chain reaction procedure
FIRRAO, Giuseppe;GOBBI, Emanuela;LOCCI, Romano
1994-01-01
Abstract
The rapid diagnosis of apple proliferation mycoplasma-like organism, using polymerase chain reaction amplification of part of the 16S rRNA gene is reported. Apple proliferation MLO can be detected in as little as 0.3 g of diseased tissue. Results compare favourably with those obtained by other PCR-based methods. Operational speed, sensitivity, specificity and ease of performance of the method, with particular reference to indexing purposes, are discussed.File in questo prodotto:
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