The catalytic subunit of a membrane-bound pyrophosphatase was purified by electroendosmotic preparative electrophoresis from etiolated pea stem mitochondria. The enzyme was identified as a single peak relatively pure, because only a very limited number of polypeptides were detectable by SDSlPAGE of the active fractions. The pyrophosphatase was associated to a band with a molecular mass of 35 kDa, showing a specific activity of 0.7 pmol P, ' mg-' protein . min-' (37"C, pH 8.0) and an apparent K,,, value of 200 pM. The hydrolytic activity required Mgz+, was inhibited by imidodiphosphate (HNO,P,Na,), Ca", F- and was stimulated by phospholipids. Cardiolipin, phophatidylcholine and phosphatidylethanolamine had the maximal activating effect. The isolated protein is very similar to the catalytic subunit of pyrophosphatases isolated from rat liver @-subunit) and Saccharomyces cerevisiae mitochondria.

Isolation of the catalytic subunit of a membrane-bound H+-pyrophosphatase from pea stem mitochondria

ZANCANI, Marco;MACRI', Francesco Arturo;DAL BELIN PERUFFO, Angelo;VIANELLO, Angelo
1995

Abstract

The catalytic subunit of a membrane-bound pyrophosphatase was purified by electroendosmotic preparative electrophoresis from etiolated pea stem mitochondria. The enzyme was identified as a single peak relatively pure, because only a very limited number of polypeptides were detectable by SDSlPAGE of the active fractions. The pyrophosphatase was associated to a band with a molecular mass of 35 kDa, showing a specific activity of 0.7 pmol P, ' mg-' protein . min-' (37"C, pH 8.0) and an apparent K,,, value of 200 pM. The hydrolytic activity required Mgz+, was inhibited by imidodiphosphate (HNO,P,Na,), Ca", F- and was stimulated by phospholipids. Cardiolipin, phophatidylcholine and phosphatidylethanolamine had the maximal activating effect. The isolated protein is very similar to the catalytic subunit of pyrophosphatases isolated from rat liver @-subunit) and Saccharomyces cerevisiae mitochondria.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11390/674984
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