The production of superoxide radical (O-2(.-)) was studied in plasma membrane vesicles isolated by aqueous polymer two-phase partitioning from roots of zinc-sufficient and zinc-deficient bean (Phaseolus vulgaris L. cv. Prelude) plants. The two populations of Vesicles were highly enriched in plasma membrane and had similar composition as evidenced by the specific membrane marker enzymes. Vesicles from zinc-deficient roots showed higher rates of NAD(P)H oxidation compared to vesicles from zinc-sufficient plants. The NAD(P)H-dependent formation of O-2(.-) in plasma membrane Vesicles was also highly increased by zinc deficiency. For both activities, a higher response to zinc deficiency was observed when NADPH was used as electron source. Re-supply of zinc to deficient plants for 24 h substantially decreased the rates of NAD(P)H oxidation and O-2(.-) production in isolated vesicles. The NADPH-dependent O-2(.-) generation was strongly stimulated by FAD and showed a high pH optimum; it was scarcely affected by Triton X-100 or even inhibited in the presence of FAD and was almost insensitive to Antimycin A. The results suggest the presence at the plasma membrane of bean roots of an O-2(.-) generating activity, preferentially utilizing NADPH, which is affected by the zinc nutritional status of the plant. This finding, together with previous observations on cytosolic and microsomal fractions prepared from zinc-deficient roots of different plants, is consistent with a role of zinc in membrane stabilization by controlling the level of oxidizing O-2 species.
ZINC-DEFICIENCY ENHANCED NAD(P)H-DEPENDENT SUPEROXIDE RADICAL PRODUCTION IN PLASMA-MEMBRANE VESICLES ISOLATED FROM ROOTS OF BEAN-PLANTS
PINTON, Roberto;
1994-01-01
Abstract
The production of superoxide radical (O-2(.-)) was studied in plasma membrane vesicles isolated by aqueous polymer two-phase partitioning from roots of zinc-sufficient and zinc-deficient bean (Phaseolus vulgaris L. cv. Prelude) plants. The two populations of Vesicles were highly enriched in plasma membrane and had similar composition as evidenced by the specific membrane marker enzymes. Vesicles from zinc-deficient roots showed higher rates of NAD(P)H oxidation compared to vesicles from zinc-sufficient plants. The NAD(P)H-dependent formation of O-2(.-) in plasma membrane Vesicles was also highly increased by zinc deficiency. For both activities, a higher response to zinc deficiency was observed when NADPH was used as electron source. Re-supply of zinc to deficient plants for 24 h substantially decreased the rates of NAD(P)H oxidation and O-2(.-) production in isolated vesicles. The NADPH-dependent O-2(.-) generation was strongly stimulated by FAD and showed a high pH optimum; it was scarcely affected by Triton X-100 or even inhibited in the presence of FAD and was almost insensitive to Antimycin A. The results suggest the presence at the plasma membrane of bean roots of an O-2(.-) generating activity, preferentially utilizing NADPH, which is affected by the zinc nutritional status of the plant. This finding, together with previous observations on cytosolic and microsomal fractions prepared from zinc-deficient roots of different plants, is consistent with a role of zinc in membrane stabilization by controlling the level of oxidizing O-2 species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
