After amplification and purification, PCR products from the iap gene of different Listeria monocytogenes serovars were subjected to sequencing to define the intraspecific differences within the gene encoding for the associated invasive protein. Nucleotide sequence analysis of the tested serovars demonstrated a division into serovar-groups obtained by using restriction enzyme analysis and single strand conformation polymorphism. Three groups were identified: the first included serovars 1/2a and 3a, the second serovars 1/2b, 3b and 4b, and the third serovar 1/2c.

Sequence Analysis of a PCR product from the iap Gene of Listeria monocytogenes serovars most frequently isolated from Food

MANZANO, Marisa;COMI, Giuseppe
2000-01-01

Abstract

After amplification and purification, PCR products from the iap gene of different Listeria monocytogenes serovars were subjected to sequencing to define the intraspecific differences within the gene encoding for the associated invasive protein. Nucleotide sequence analysis of the tested serovars demonstrated a division into serovar-groups obtained by using restriction enzyme analysis and single strand conformation polymorphism. Three groups were identified: the first included serovars 1/2a and 3a, the second serovars 1/2b, 3b and 4b, and the third serovar 1/2c.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11390/717846
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact