A simple procedure for the processing of MLO (mycoplasma-like organisms)-infected plant samples for PCR analysis is described. It is based on differential centrifugation and removal of enzyme inhibiting agents with the aid of a resin. Results of the amplifications are comparable with those achieved with more complex DNA extraction procedures. The method does not require any extraction with organic solvents or alcoholic precipitation of nucleic acids.
RAPID PREPARATION OF DNA FROM PHYTOPATHOGENIC MYCOPLASMA-LIKE ORGANISMS FOR POLYMERASE CHAIN-REACTION ANALYSIS
FIRRAO, Giuseppe;LOCCI, Romano
1993-01-01
Abstract
A simple procedure for the processing of MLO (mycoplasma-like organisms)-infected plant samples for PCR analysis is described. It is based on differential centrifugation and removal of enzyme inhibiting agents with the aid of a resin. Results of the amplifications are comparable with those achieved with more complex DNA extraction procedures. The method does not require any extraction with organic solvents or alcoholic precipitation of nucleic acids.File in questo prodotto:
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