We have isolated 44 SSRs from an AC-enriched genomic library from almond (Prunus amygdalus Batsch.). Twenty SSRs were screened for their polymorphism in 16 cultivars and for their transportability in seven different Prunus species (peach, nectarine, apricot, European plum, Japanese plum, sweet cherry, sour cherry) and in apple. The expected heterozygosity ranged from 0.62 to 0.89. About 30% of primers gave successful amplification in seven different Prunus species; in two cases amplifications were obtained also in apple.
Microsatellites isolated in Almond from an ac-repeat enriched library
TESTOLIN, Raffaele;MESSINA, Rachele;LAIN, Orietta;MARRAZZO, Maria Teresa;CIPRIANI, Guido
2004-01-01
Abstract
We have isolated 44 SSRs from an AC-enriched genomic library from almond (Prunus amygdalus Batsch.). Twenty SSRs were screened for their polymorphism in 16 cultivars and for their transportability in seven different Prunus species (peach, nectarine, apricot, European plum, Japanese plum, sweet cherry, sour cherry) and in apple. The expected heterozygosity ranged from 0.62 to 0.89. About 30% of primers gave successful amplification in seven different Prunus species; in two cases amplifications were obtained also in apple.File in questo prodotto:
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2004 MICROSATELLITES ISOLTED IN ALMOND.pdf
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