An HPLC method suitable for rapid triacylglycerol analysis by revers phase elution is describe. A Spherisorb column ( 15 cm x 4,6 mm I.D.) with 3 um particle size and C18 stationary phace with 12% carbon loading was used. Alution was scared out under isocratic conditions ( acetone, aceto nitrile and Diethyl ether were used as solvent mixure) and Light Scattering Detector was employed. In addition to being rapid ( 15 min for olive oil) the proposed method shows good repeatability and it may represent suitable technique to charaterize addible fact substances and to determine standards for quality control of fact containing foods.
Rapid HPLC analysis of triacylglycerols by isocratic elution and light scattering detection
CONTE, Lanfranco;
1992-01-01
Abstract
An HPLC method suitable for rapid triacylglycerol analysis by revers phase elution is describe. A Spherisorb column ( 15 cm x 4,6 mm I.D.) with 3 um particle size and C18 stationary phace with 12% carbon loading was used. Alution was scared out under isocratic conditions ( acetone, aceto nitrile and Diethyl ether were used as solvent mixure) and Light Scattering Detector was employed. In addition to being rapid ( 15 min for olive oil) the proposed method shows good repeatability and it may represent suitable technique to charaterize addible fact substances and to determine standards for quality control of fact containing foods.File in questo prodotto:
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