A simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal.
Continuous monitoring of the biliary excretion of a pulse of labeled compounds
SORRENTINO, Dario Rosario
1988
Abstract
A simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
