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EnglishA simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal.
| Titolo: | Continuous monitoring of the biliary excretion of a pulse of labeled compounds |
| Autori: | |
| Data di pubblicazione: | 1988 |
| Rivista: | |
| Abstract: | A simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal. |
| Handle: | http://hdl.handle.net/11390/675810 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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