CINECA IRIS Institutional Research Information System
IRIS è la piattaforma adottata dall’Università degli Studi di Udine per gestire l’archivio aperto dei prodotti della ricerca. L’archivio contiene articoli, contributi in volume, monografie, interventi pubblicati in atti di convegno, Tesi di dottorato e altri materiali.
EnglishA simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal.
| Titolo: | Continuous monitoring of the biliary excretion of a pulse of labeled compounds |
| Autori: | |
| Data di pubblicazione: | 1988 |
| Rivista: | |
| Abstract: | A simple method allowing the continuous monitoring of the biliary excretion of a labelled, pulse-injected compound in a single-pass perfused rat liver is described. The collection device is a mini-strip-chart recorder: bile is collected on its moving paper as a uniform trail that dries almost instantaneously. After rapid injection into the portal vein, the biliary excretion of a labelled compound can be assessed measuring the radioactivity on the paper by cutting and counting narrow strips of paper or by a radiochromatogram scanner. Experiments performed with [14C]-bilirubin, [3H]-taurocholate, and [14C]-nitrofurazone attest the validity of this technique, which can potentially improve the accuracy of hepatic transport studies in the isolated organ as well as in the intact animal. |
| Handle: | http://hdl.handle.net/11390/675810 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
File in questo prodotto:
http://hdl.handle.net/11390/675810
Copyright © 2021