Crude extracts obtained from the edible shoots of Cicerbita alpina using microwave-assisted extraction have been qualitatively profiled by liquid chromatography coupled with an ion trap mass spectrometry detector and an electrospray ionization interface (LC/ESI-MS3) for their phenolic content. The main challenge of the present investigation was to create a working strategy designed to obtain a rich phenolic profile despite the limited amount of starting plant material and phytochemical data available. The best extraction conditions (temperature 90 °C; time 5 min; solvent methanol:water 50:50; sample weight 3 g) were achieved using a full factorial 24 experimental design. Fifteen compounds, including flavonoid conjugates and phenolic acid derivatives, were detected and tentatively identified. The total phenolic content varied from 93.58 mg g−1 gallic acid equivalents (GAE), for the cultivated plant to 10.54 mg g−1 GAE for the wild one, whereas the total flavonoid content varied from 145.00 mg g−1 rutin for the cultivated plant to 25.22 mg g−1 rutin for the wild one. A total of 11 compounds are herein reported, for the first time, as coming from this plant source. © 2013 Society of Chemical Industry
Microwave-assisted extraction of edible Cicerbita alpina shoots and its LC-MS phenolic profile
ALEXANDRU, Lavinia;PIZZALE, Lorena;CONTE, Lanfranco;
2013-01-01
Abstract
Crude extracts obtained from the edible shoots of Cicerbita alpina using microwave-assisted extraction have been qualitatively profiled by liquid chromatography coupled with an ion trap mass spectrometry detector and an electrospray ionization interface (LC/ESI-MS3) for their phenolic content. The main challenge of the present investigation was to create a working strategy designed to obtain a rich phenolic profile despite the limited amount of starting plant material and phytochemical data available. The best extraction conditions (temperature 90 °C; time 5 min; solvent methanol:water 50:50; sample weight 3 g) were achieved using a full factorial 24 experimental design. Fifteen compounds, including flavonoid conjugates and phenolic acid derivatives, were detected and tentatively identified. The total phenolic content varied from 93.58 mg g−1 gallic acid equivalents (GAE), for the cultivated plant to 10.54 mg g−1 GAE for the wild one, whereas the total flavonoid content varied from 145.00 mg g−1 rutin for the cultivated plant to 25.22 mg g−1 rutin for the wild one. A total of 11 compounds are herein reported, for the first time, as coming from this plant source. © 2013 Society of Chemical IndustryI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
