Freshly-extracted apple juice was exposed to high pressure carbon dioxide (HP-CO2) treatment at 20, 35 and 45 °C at different pressure conditions (6.0, 12.0 and 18.0 MPa) for up to 30 min. Samples were analysed for residual enzymatic activity. The time needed for 90% enzyme inactivation (Dp) decreased when CO2 pressure increased, while the CO2 pressure sensitivity of the enzyme (zp) showed no variation with temperature. The HP-CO2 treatment at 12 MPa and 35 °C allowed the minimum residual enzyme activity (20%) to be reached in 10 min. Samples treated under these conditions showed lower polyphenoloxidase activity and higher microbial stability than untreated apple juice while presenting a sensory fresh-likelihood higher than thermally pasteurized apple juice. © 2016 Elsevier Ltd