The effect of high pressure homogenisation (HPH) on decontamination, protein structure (turbidity, particle size, free sulfhydryl groups, protein electrophoretic mobility) and selected functional properties (viscosity, foaming and gelling properties, immunoreactivity) of egg white were evaluated. HPH at 150 MPa for multiple passes allowed the progressive inactivation of S. enterica SDMZ 9898. In addition, HPH modified egg white proteins by inducing unfolding and aggregation phenomena. The latter would occur by means of hydrophobic interactions among partially unfolded proteins. Protein structure modifications induced by HPH were not sufficient to modify egg white foaming properties. Depending on the intensity of the process, HPH allowed to modify apparent viscosity of egg white and firmness of egg white gel. HPH decreased egg white immunoreactivity probably due to protein epitopes hiding upon aggregation.

Effect of high pressure homogenisation on microbial inactivation, protein structure and functionality of egg white

PANOZZO, Agnese;MANZOCCO, Lara;CALLIGARIS, Sonia;MAIFRENI, Michela;LIPPE, Giovanna;NICOLI, Maria Cristina
2014-01-01

Abstract

The effect of high pressure homogenisation (HPH) on decontamination, protein structure (turbidity, particle size, free sulfhydryl groups, protein electrophoretic mobility) and selected functional properties (viscosity, foaming and gelling properties, immunoreactivity) of egg white were evaluated. HPH at 150 MPa for multiple passes allowed the progressive inactivation of S. enterica SDMZ 9898. In addition, HPH modified egg white proteins by inducing unfolding and aggregation phenomena. The latter would occur by means of hydrophobic interactions among partially unfolded proteins. Protein structure modifications induced by HPH were not sufficient to modify egg white foaming properties. Depending on the intensity of the process, HPH allowed to modify apparent viscosity of egg white and firmness of egg white gel. HPH decreased egg white immunoreactivity probably due to protein epitopes hiding upon aggregation.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11390/1020146
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